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Effect of BacA and LpsB on the S. meliloti LPS. Cultures of the wild-type strain Rm1021 and the defined isogenic mutants were grown on LB plates and LPS extractions were performed exactly as described in Materials and Methods by using either an SDS lysis (a) or hot water-phenol method (b and c). S-LPS and R-LPS refer to smooth and rough LPS, respectively.
