TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| pMU720 | Gal, IncB, miniplasmid | 14 |
| pMU525 | lac′ZYA′, Tp, IncW, low-copy-number translational fusion vector | 28 |
| pMU1550 | repA-lacZ translational fusion carrying nt 1-789 of pMU720; Tp, IncW, IncB | 29 |
| pMU1578 | repB-lacZ translational fusion carrying nt 1-730 of pMU720; Tp, IncW, IncB | 29 |
| pIZ513 | pACYC184 derivative carrying the specialized rrnB operon under the control of tac promoter and a lacIq gene; Cm | 19 |
| pIZ514 | pACYC184 derivative carrying a deletion mutant of the specialized rrnB operon under the control of tac promoter and a lacIq gene; Cm | 19 |
a
Abbreviations: Cm, chloramphenicol resistance; Tp, trimethoprim resistance; Gal, ability to promote fermentation of galactose. Mutations introduced into the repA-lacZ and repB-lacZ fusion plasmids are described in Results.