FIG. 1.

Structure of pruR-putP loci of PAO strains and plasmids. Gene names (PA) correspond to annotation of the Pseudomonas Genome Project (www.pseudomonas.com). DNA cassettes, Tn5-751, and histidine- codon tag sequences are not drawn to scale. Thin solid and broken lines indicate chromosomal and plasmid sequences, respectively. Strains PAO4502, PAO4528, and PAO4547 carry FRT-Gm, FRT and ΩSp/Sm cassettes on putA, respectively. Strain PAO4534 has FRT and FRT-Gm inserts on putA and pruR, respectively. Only relevant restriction sites are indicated: E, EcoRI; Ba, BamHI; Bb, BbsI; Bg, BglI; Bn, BanIII; K, KpnI; Pm, PmaCI; Sa, SalI; Sc, SacI; Su, Sau3AI; Sm, SmaI; Xh, XhoI.