TABLE 2.
Regulation of pruA′-′lacZ fusion by PutA and PruRa
| Enzyme source | β-Galactosidase activity (102 Miller units)b
|
||||
|---|---|---|---|---|---|
| Glu | Pro | Glu + Pro | Pro + Suc | Pro + NH4 | |
| PAO1 (wild type) | <0.1 | 62 ± 1 | 49 ± 2 | 66 ± 2 | 59 ± 2 |
| PAO4502 (putA::FRT-Gm) | <0.1 | NGc | 96 ± 6 | NG | NG |
| PAO6087 (pruR::Tn5-751) | <0.1 | NG | <0.1 | NG | NG |
| PAO4534 (pruR::FRT-Gm putA::FRT) | <0.1 | NG | <0.1 | NG | NG |
a
Cells harboring pYI378 (putAPAO′::′lacZ) were grown in MMP medium supplemented with 20 mM carbon and nitrogen sources.
b
Activities of β-galactosidase were measured in cell extracts prepared as described previously (26, 28) and are represented as averages of three measurements with standard errors. Carbon and nitrogen sources added to MMP medium: Glu, glutamate; Pro, proline; Suc, succinate.
c
NG, no growth or very poor growth (see Fig. 4).