Skip to main content
. 2002 Oct;184(20):5814–5817. doi: 10.1128/JB.184.20.5814-5817.2002

FIG. 2.

FIG. 2.

Recombinant production and cellular localization of P. haloplanktis TAB23 α-amylase (precursor and mature forms) in P. haloplanktis TAC125. (A) Schematic representation of gene constructs directing the production of the α-amylase precursor (pFFamy) and its mature form (pFFamyΔCt). Pasp, P. haloplanktis TAC125 aspC transcriptional promoter; SP, signal peptide; C-term, C-terminal propeptide. (B) Western blot analysis of cell extracts (lanes 1 and 3) and supernatants (lanes 2 and 4) of pFFamy (lanes 1 and 2) and pFFamyΔCt (lanes 3 and 4) recombinant P. haloplanktis TAC125 cells. The analyzed samples came from cultures at the same growth phase and from equal amount of cells. The immunodetection was performed by using anti-C-terminal polyclonal antiserum as described in reference 8.