FIG. 3.

Secretion kinetics of recombinant precursor and mature α-amylase forms in recombinant P. haloplanktis TAC125 cells. Kinetics of bacterial growth (upper panel), recombinant α-amylase (middle panel), and alkaline phosphatase (lower panel) activities in the cell-free supernatants of P. haloplanktis TAC125 cells transformed with pFFamy (*) and pFFamyΔCt (▪) are presented. Enzyme activities are expressed as percentages of the maximal activity recorded in the cell-free supernatants. The curves were constructed from average results of three independent experiments at 4°C.