TABLE 1.
Bacterial strains and plasmids used in this work
| Plasmid or strain | Description | Reference |
|---|---|---|
| Plasmids | ||
| pUCL | Vector deriving from the AflIII/EcoRI digestion of pUC18, fill-in reaction of the sticky ends and ligation; NdeI disruption | This work |
| Clone Q | pGEM-4z containing the T/R box | 18 |
| pFF | PUCL containing the T/R box, the promoter and termination region of the P. haloplanktis TAC125 aspC gene | This work |
| pαH12 | pUC12 derivative containing the P. haloplanktis α-amylase gene | 8 |
| pαH12wt* | pUC12 derivative containing the P. haloplanktis α-amylase gene devoid of the gene region encoding the C-terminal domain | 8 |
| pFFamy | pFF containing the pαH12 insert | This work |
| pFFamyΔCt | pFF containing the pαH12wt∗ insert | This work |
| Bacterial strains | ||
| TAD1 | Psychrobacter sp. strain TAD1 | 6 |
| TAB23 | P. haloplanktis TAB23 (formerly Alteromonas haloplanktis A23) | 9 |
| TAC125 | P. haloplanktis TAC125 | 3 |
| DH5α | E. coli [supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1] | 12 |
| S17-1(λpir) | E. coli strain S17-1 (λpir) [thi pro hsd(r− m+) recA::RP4-2-Tcr::Mu Kmr::Tn7 Tpr Smr λpir] | 17 |