TABLE 1.
End point titration of HIV-infected cell culture supernatants
| Culture supernatant | Titer of p24 Ag in pg/ml (pg of HIV Ag/ml)a | Sub- or serotype | Test result, expressed as index (signal/cutoff value)b |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VIDAS HIV DUO Ultra | Murex HIV Ag-Ab combination | Cobas Core HIV Combi | VIDAS HIV DUO | Genscreen Plus HIV Ag-Ab | Enzygnost Integral | Vironostika HIV Uniform II Ag-Ab | Cobas Core HIV Ag EIA | Elecsys HIV Ag | Coulter HIV-1 p24 Ag | |||
| MP33 | 8 (29) | A | 2.28 | 0.96 | 1.14 | 0.76 | 0.34 | 0.12 | 0.55 | 2.74 | 2.09 | 2.48 |
| MP77 | 5 (18) | B | 1.12 | 0.60 | 0.61 | 0.60 | 0.41 | 0.10 | 0.53 | 1.28 | 1.34 | 1.59 |
| MP37 | 6 (22) | C | 1.96 | 1.16 | 0.52 | 0.48 | 0.31 | 0.05 | 0.54 | 1.50 | 1.65 | 1.49 |
| VI824 | 11 (40) | D | 3.04 | 1.20 | 1.41 | 0.80 | 0.39 | 0.17 | 0.49 | 3.25 | 2.76 | 2.92 |
| MP48 | 5 (18) | E (CRF A/E) | 1.24 | 0.52 | 0.67 | 0.56 | 0.32 | 0.10 | 0.55 | 1.39 | 1.47 | 0.58 |
| VI1310 | 9 (33) | F | 1.24 | 1.19 | 1.14 | 0.60 | 0.44 | 0.31 | 0.54 | 2.59 | 2.24 | 1.81 |
| VI1197 | 8 (29) | G | 1.72 | 1.20 | 0.95 | 0.60 | 0.28 | 0.28 | 0.56 | 2.19 | 1.93 | 0.66 |
| VI991 | 6 (22) | H | 0.2 | 1.98 | 1.23 | 0.32 | 0.34 | 0.13 | 0.50 | 2.97 | 1.53 | 1.84 |
| MP331 | 6 (22) | O | 0.68 | 1.16 | 0.88 | 0.40 | 0.29 | 0.10 | 0.52 | 2.12 | 1.65 | 0.47 |
| VI390 | 15 (54) | HIV-2 | 0.36 | 1.65 | 0.44 | 0.24 | 0.15 | 0.06 | 0.47 | 1.80 | 3.06 | 0.12 |
a
Calibrated with Dupont standard material (1 pg of p24 Ag corresponds to 3.6 pg of total HIV Ag).
b
A value of ≥1 is considered positive.