TABLE 2.
Interassay reproducibility of the LightCycler assay in either a whole-blood DNA background or in watera
| No. of copies | Water background
|
Whole-blood DNA background
|
||
|---|---|---|---|---|
| Mean Ct value (SD) | Mean copy no. (SD) | Mean Ct value (SD) | Mean copy no. (SD) | |
| 10,000 | 27.34 (1.34) | 11,893 (4,771) | 27.22 (0.60) | 9,909 (1,769) |
| 1,000 | 30.84 (1.44) | 1,292 (286) | 30.69 (0.77) | 962 (198) |
| 100 | 35.25 (2.33) | 123 (88) | 35.00 (2.18) | 71 (44) |
| 10 | 40.65 (3.60) | 20 (27) | 40.28 (5.56) | 11 (13) |
a
For each determination, three independent experiments were performed in quadruplicate.