TABLE 2.
Sensitivities of different HIV-1 group O proteases to indinavir and saquinavir using the lambda-based phenotypic method and the standard in vitro drug susceptibility assay
| Method | Sample (mutation pattern) | IC50 (nM) (fold increase) of:
|
|
|---|---|---|---|
| Indinavir | Saquinavir | ||
| Lamda phage | HXB2 (WTa) | 25 ± 2 | 2.57 ± 0.88 |
| ESP2-0 (WT) | 28 ± 5.75 | 5.08 ± 0.41 | |
| ESP2-4 (L10V, G48M, F53L, 154V) | 150 ± 32.25 (5,4) | 122.67 ± 39.5 (24) | |
| Standard cell culture assay | HXB2 | NDb | ND |
| ESP1-2 (WT) | 15.13 | 5 ± 0.78 | |
| ESP2-3 (L10V, G48M, F53L, 154V) | 185.5 ± 53.5 (12) | 1,264.7 ± 285 (> 200) | |
a
WT, wild type.
b
ND, not done.