TABLE 2.
Evaluation of assays with recombinant POMP90 fragments with experimental seraa
| Test | Sensitivity (%) with group 1 sera | Specificity (%) with group 2 sera | No. of positive samples in group 3
|
|
|---|---|---|---|---|
| Arth/conj | Enteric | |||
| rOMP90-1 ELISA | 47.1 | 96.9 | 1 | 0 |
| rOMP90-2 ELISA | 32.9 | 100 | 2 | 0 |
| rOMP90-3 ELISA | 95.7 | 100 | 0 | 0 |
| rOMP90-4 ELISA | 94.3 | 100 | 0 | 0 |
| rOMP90-5 ELISA | 91.4 | 98.4 | 3 | 1 |
| rOMP90-6 ELISA | 91.4 | 96.9 | 2 | 0 |
| rOMP90-7 ELISA | 72.9 | 96.9 | 0 | 0 |
| rOMP90-8 ELISA | 78.6 | 98.4 | 2 | 0 |
| rOMP90-11 ELISA | 2.9 | 100 | 3 | 0 |
| rOMP91B iELISA | 95.7 | 98.4 | 1 | 0 |
| CFT | 91.4 | 98.4 | 4 | 0 |
a
Group 1, sera from experimentally infected ewes (n = 70); group 2, sera from OEA-free free flocks (n = 64); group 3, sera from SPF lambs vaccinated with arthritogenic or conjunctival (Arth/conj; n = 5) or enteric (n = 4) subtypes of C. pecorum. Sensitivity = [number of true positives/(number of true positives + number of false negatives)] × 100; specificity = [number of true negatives/(number of true negatives + number of false positives)] × 100.