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. 2002 Nov;40(11):3956–3963. doi: 10.1128/JCM.40.11.3956-3963.2002

FIG. 1.

FIG. 1.

Quantitative analysis of fluorescence signal during real-time PCR with the lef primer-probe set and serial dilutions of the target. (A) Amplification of serial dilutions of purified B. anthracis Carbosap DNA. Dilutions of DNA ranged from 3 to 0.0003 ng. ◊, 3 ng; ⧫, 1 ng; ○, 0.3 ng; ×, 0.1 ng; ▪, 0.03 ng; small ⋄, 0.01 ng; ▪, 0.003 ng; •, 0.001 ng; smaller ⋄, 0.0003 ng; line at the bottom, negative control (no DNA). (B) Amplification of serial dilutions of nonlysed B. anthracis Carbosap spores. Dilutions ranged from 20,000 to 2 spores. ◊, 20,000 spores; ⧫, 2,000 spores; ▪, 200 spores; ×, 20 spores; small ◊, 2 spores; ▪, positive control (1 ng of B. anthracis Carbosap DNA; line, negative control (no DNA). (C) Amplification of stationary-phase cultures inoculated with serial dilutions of B. anthracis Carbosap spores. Dilutions of inocula ranged from 30,000 to 3 spores. ◊, 30,000 spores; ⧫, 3,000 spores; ▪, 300 spores; ×, 30 spores; small ◊, 3 spores; ▪, positive control (1 ng of B. anthracis Carbosap DNA); line at the bottom, negative control (no DNA). The inset in panel A refers to the standard curve of the amplification reactions on serial dilutions reported in panel A (the regression coefficient is 1, as calculated with LightCycler software [version 3.0]).