TABLE 2.

Primers and probes used in real-time PCR

Name	Target	Usea	Sequence (5′-3′)	GenBank accession no.	Position	Tm (°C)
OG132	lef	Primer	TTTTTATGACAAGAAATATTGCCTTT	M29081	372-397	58.0
OG133	lef	Primer	CAGCTTCCTCCCCTTTTACTTC	M29081	718-739	62.0
OG134	lef	Probe Red 705	TGTACAGGGGGCGGGCGGTC	M29081	544-561	72.8
OG135	lef	Probe FL	TTGAGTGGTCCCGTCTTTATCCCCC	M29081	570-589	69.3
OG136	rpoB	Primer	TTGCTTGAAATTTATGAGCGTCTAC	AF205323	285-309	61.5
OG137	rpoB	Primer	ATTGTTCCTTCTGCCGCTAAAA	AF205323	493-514	62.2
OG138	rpoB	Probe Red 640	TGTAGGTCGCTACAAGATCAACAAG	AF205323	395-419	64.0
OG140	rpoB	Probe FL	AAGCGCTATGATTTAGCAA	AF205323	375-393	56.2
OG144	lefC	Primer	TATGTCATCTTTCTTTGGCTCAATAG	AJ413935	936-911	60.7
FM22	lefC	Primer	TTTATGCACCGGAAGCTTTTAA	AJ413935	719-740	60.2
FM23	lefC	Probe FL	CAACGGATGCTGTCAAGATATG	AJ413935	799-820	60.8
FM24	lefC	Probe Red 640	AAATGGGAAAAGATAAAACAGCACTATCA	AJ413935	823-851	63.4

^aProbe Red 705, downstream probe marked with reporter dye Red 705; probe FL, upstream probe labeled with fluorescein; probe Red 640, downstream probe marked with reporter dye Red 640.