Abstract
A direct, noninvasive method of assessing the oxidation-reduction potential of an intramyocardial respiratory chain component is described. The technique is based on the differences in spectral properties between the oxidized and reduced forms of nicotinamide adenine dinucleotide (NADH). The tissue surface fluorescence from intracellular NADH may be measured and documented photographically. Noose occlusion of a coronary artery produced detectable NADH fluorescence in 15 seconds in the subtended ischemic epicardium. This fluorescence of reduced pyridine nucleotide resolved following 60 seconds of reperfusion of the ischemic myocardium. The reduction of epicardial NADH with ischemia is a rapid and reversible process. A subsequent noose reocclusion resulted in a reproducible pattern of fluorescence. The technique of NADH fluorescence photography appears superior to current methods of assessing tissue oxygen supply:demand.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barlow C. H., Chance B. Ischemic areas in perfused rat hearts: measurement by NADH fluorescence photography. Science. 1976 Sep 3;193(4256):909–910. doi: 10.1126/science.181843. [DOI] [PubMed] [Google Scholar]
- CHANCE B., BALTSCHEFFSKY H. Respiratory enzymes in oxidative phosphorylation. VII. Binding of intramitochondrial reduced pyridine nucleotide. J Biol Chem. 1958 Sep;233(3):736–739. [PubMed] [Google Scholar]
- CHANCE B., COHEN P., JOBSIS F., SCHOENER B. Intracellular oxidation-reduction states in vivo. Science. 1962 Aug 17;137(3529):499–508. doi: 10.1126/science.137.3529.499. [DOI] [PubMed] [Google Scholar]
- Chance B., Salkovitz I. A., Kovach A. G. Kinetics of mitochondrial flavoprotein and pyridine nucleotide in perfused heart. Am J Physiol. 1972 Jul;223(1):207–218. doi: 10.1152/ajplegacy.1972.223.1.207. [DOI] [PubMed] [Google Scholar]
- HUCKABEE W. E. Abnormal resting blood lactate. I. The significance of hyperlactatemia in hospitalized patients. Am J Med. 1961 Jun;30:840–848. doi: 10.1016/0002-9343(61)90172-3. [DOI] [PubMed] [Google Scholar]
- Harken A. H. Lactic acidosis. Surg Gynecol Obstet. 1976 Apr;142(4):593–606. [PubMed] [Google Scholar]