FIG. 1.

Production and purification of recombinant HHV-6 U94/REP. E. coli K-12 was lysed before induction (n.i.) or after induction by IPTG for 1, 3, and 5 h. Aliquots of bacterial extract were separated by SDS-PAGE and stained with Coomassie blue. The recombinant protein was recovered in the insoluble fraction of the total extract (arrow). Following a purification step by ion-exchange chromatography, a highly purified U94/REP was obtained, as shown in the right panel. Sizes of molecular mass markers (MW) are shown.