FIG. 1.

Dot blot hybridization of membrane-blotted RNA of trypanosomes and various controls using anti-fluorescein alkaline phosphatase-labeled PNA probes Tbr7, Tbr16, and Tbr18 and positive control EU/uni2. Hybridization reaction is revealed by addition of chemiluminescent substrate. Isolates shown (indicated by lane letter and row letter) are as follows: Aa, RNA of T. evansi; Ba, T. congolense; Ca, T. brucei brucei; Da, T. brucei rhodesiense; Ea, T. brucei gambiense; Fa, T. equiperdum; Ab, P. falciparum; Bb, L. donovani; Cb, human blood sample containing trypanosomes; Db, E. coli; Eb, negative control in which RNA content was omitted; Fb, S. cerevisiae.