PFGE banding patterns after SpeI digestion. Lanes 1 to 3, VIM-1-producing P. putida isolates from the general ICU (lane 1, isolate VA-304/99; lane 2, isolate VA-523/99; lane 3, isolate VA-420/00); lane 4, multidrug-resistant P. putida isolate from the nephrology department; lanes 5 and 6, two antibiotic-susceptible P. putida isolates obtained from the general ICU at the same hospital in 2000; lane L, λ phage DNA concatemers as a size marker.