FIG. 2.

Effect of S535A eIF2B on apoptosis induced by PI 3-kinase inhibition or serum deprivation. Cells were cotransfected with expression plasmids for wild-type or S535A mutant eIF2B or for dominant-negative PI 3-kinase (dnPI3K) plus pEGFP-C1. Two days after transfection, cells were either left untreated, treated with 50 μM LY294002, or deprived of serum for 24 h; then they were fixed and stained with Hoechst dye. Transfected cells were identified by GFP fluorescence and scored for apoptosis by nuclear morphology. Data are averaged from three independent experiments.