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S535A eIF2B does not affect apoptosis induced by cycloheximide. Cells were transfected with a control, wild-type eIF2B, or mutant S535A eIF2B expression plasmid plus the GFP expression plasmid pEGFP-C1. Two days after transfection, cells were treated with 10 μg of cycloheximide (CHX)/ml for 24 h and then stained with Hoechst dye, and GFP-positive cells were scored for apoptosis by nuclear morphology. Data are averaged from three independent experiments.
