FIG. 8.
Inhibition of translation induces cytochrome c release. (A) A total of 105 PC12 cells were plated on poly-l-lysine-treated coverslips in 35-mm plates and treated the following day with either LY294002 (50 μM; middle panel) or cycloheximide (10 μg/ml; right panel) for 6 h. (Left panel) Control. Cells were analyzed for cytochrome c release by anti-cytochrome c immunostaining, and representative fields were photographed with a confocal microscope. (B) Cells were treated for the indicated times with LY294002 or cycloheximide (CHX) and analyzed for cytochrome c release by immunostaining. Data are averaged from three independent experiments.