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Recovery of methylation after 5-aza-CdR treatment. The indicated cell types were treated for 24 h with 3 × 10−7 M 5-aza-CdR; then the medium was changed, and the cells were propagated further. DNA was extracted at the times indicated after the drug was added to the cultures, and the methylation statuses of the CI-f and CII-d fragments were measured by Ms-SNuPE analysis. Results shown are average values from two separate experiments.
