FIG. 7.

Methylation kinetics of newly synthesized DNA at CI-f and CII-d. ES cells containing the genes for the indicated DNA methyltransferases were pulsed for 1 h with BrdU. The pulse was then removed, and fresh medium was added to the cells during the chase period. DNA was extracted from the cells at various times after the pulse began and was immunoprecipitated to isolate the BrdU-containing DNA. The methylation statuses of HpaII sites in CI-f and CII-d at the indicated time points were determined by quantitative Ms-SNuPE analysis. Data are mean values from two to six experiments; error bars, standard deviations.