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. 2002 Jan;22(2):644–656. doi: 10.1128/MCB.22.2.644-656.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Morphological and cell growth characteristics of Cdk4^R24C/R24C MEFs. (a to d) Phase-contrast pictures of Cdk4^R24C/R24C (a and b) and Cdk4^+/+ (c and d) cells at early passage 3 (a and c) and late passage 20 (b and d) are shown. Cdk4^+/+ cells at passage 20 underwent senescence (d) while Cdk4^R24C/R24C cells at passage 20 escaped senescence (b). Magnifications, ×4. (e to g) To test the ability of cells to form multiple layers (focus formation capacity), 10⁶ cells (passage 20) representing Cdk4^+/+ (e), Cdk4^+/R24C (f), and Cdk4^R24C/R24C (g) MEFs were plated in 10-cm-diameter dishes and grown for 2 weeks. Medium was changed every 3 days, and at the end of 2 weeks, plates were stained with Giemsa stain to determine the focus formation capacity.

FIG. 4. — Morphological and cell growth characteristics of Cdk4^R24C/R24C MEFs. (a to d) Phase-contrast pictures of Cdk4^R24C/R24C (a and b) and Cdk4^+/+ (c and d) cells at early passage 3 (a and c) and late passage 20 (b and d) are shown. Cdk4^+/+ cells at passage 20 underwent senescence (d) while Cdk4^R24C/R24C cells at passage 20 escaped senescence (b). Magnifications, ×4. (e to g) To test the ability of cells to form multiple layers (focus formation capacity), 10⁶ cells (passage 20) representing Cdk4^+/+ (e), Cdk4^+/R24C (f), and Cdk4^R24C/R24C (g) MEFs were plated in 10-cm-diameter dishes and grown for 2 weeks. Medium was changed every 3 days, and at the end of 2 weeks, plates were stained with Giemsa stain to determine the focus formation capacity.