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. 2002 Jan;22(2):680–691. doi: 10.1128/MCB.22.2.680-691.2002

FIG. 7.

FIG. 7.

Caspase inhibition reduces FasL expression under conditions of suppressed Akt signaling. (A) Human VSMC were preinfected with Adeno-βGal or Adeno-dnCasp-9 (MOI = 100) 12 h prior to infection with Adeno-dnAkt (MOI = 100). Cells were harvested 24 h after infection with Adeno-dnAkt. (B) VSMC were preinfected with Adeno-CrmA (MOI = 100) 12 h prior to infection with Adeno-dnAkt (MOI = 100). Cells were harvested 48 h after infection with Adeno-dnAkt. (C) VSMC were infected with an adenovirus vector expressing dnAkt (MOI = 100) in the presence or absence of the caspase inhibitors Z-VAD-FMK (20 μM) or Z-DEVD-FMK (20 μM). Cells were harvested after 48 h. Representative data from two replicate experiments are shown. Cells were detached from the culture plate with 0.5% EDTA and assayed for cell surface FasL expression by flow cytometry. Cells were incubated with anti-human FasL antibody (4H9) and then stained with FITC-conjugated anti-hamster antibody (solid curve). Hamster IgG was used as a negative control (open curve).