FIG. 8.

Adenoviral E1A reduces PDX-1:p300:BETA2 complex levels. HIT T-15 cells were transfected with the E1A (5 μg), PDX-1 (2.5 μg), and BETA2-Myc (2.5 μg) expression plasmids, as indicated. Nuclear extracts were immunoprecipitated (IP) with (A) α-p300 (RW128), (B) α-PDX-1 (N terminal), or (C) α-Myc antibody and immunoblotted (IB) with (A and B) Myc and (A and C) PDX-1 (N terminal)-specific antisera. (A) BETA2 and PDX-1 were reduced to 0.03 and 0.09 of the no-E1A-transfected control, respectively; (B) 0.16; (C) 0.15. BETA2 and PDX-1 are expressed at similar levels under these conditions (5% of total lysate [TL]).