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Complementation of Yeast snf1 by ccSNF1.
The control reference strain was MG106. snf1 denotes the yeast snf1 mutant MCY1846. The snf1 + empty vector is MCY1846 transformed with vector pVT100-U. snf1 + ccSNF1 is MCY1846 transformed with vector pSnf1c-4 (pVT100-U containing ccSNF1). SD-URA, glucose medium lacking uracil (see Methods); YPD, YP medium plus glucose (dextrose) as carbon source; YPS, YP medium plus sucrose as carbon source.
