Figure 4.

RNA Expression of Wall-Degrading Enzyme Genes in the Wild Type and in a ccsnf1 Mutant of C. carbonum.

Fungi (367-2A and T688) were grown for 7 days in liquid still culture containing, as the sole carbon source, 2% xylan (for expression of XYL1, XYL2, XYL3, XYL4, XYP1, and ARF1), 2% pectin (PGN1 and PGX1), or 2% maize cell walls (ccSNF1, MLG1, EXG1, ccRPD3, and GPD1). Total RNA was fractionated on an agarose gel and transferred to a nylon membrane, and the membrane was hybridized with probes of the indicated genes.