FIG. 3.

Lsm3p binds to pre-tRNAs. Immunoprecipitation of RNAs from a strain expressing TAP-tagged Lsm3p is shown. Lysates from the TAP-Lsm3 strain and the isogenic wild-type (WT) strain (YJV140) were immunoprecipitated with rabbit IgG agarose beads (Sigma). RNA was recovered from the lysate (T) and the immunoprecipitate (P) and analyzed by Northern hybridization. Probe names are indicated in parentheses. RNA species are shown on the right. Approximately 30-fold-more cell equivalents was loaded for the bound fractions than for the total and supernatant fractions.