FIG. 5.
Processing of tRNA precursors in the absence of Lhp1p and Lsm proteins. Shown are results of Northern analysis of tRNA processing in GAL::lsm3/lhp1-Δ and GAL::lsm5/lhp1-Δ double mutants. RNA was extracted from GAL-regulated constructs following transfer from permissive RSG medium to repressive glucose medium for the times indicated. The lhp1-Δ strain was grown on glucose medium at 30°C. Probe names are indicated in parentheses. Names of tRNAs are shown on the left, and schematic representations of tRNA species are shown on the right. tRNAPro precursors designated with a and b represent two forms of PT (32), whereas the asterisk shows the position of the shortened PT in the lhp1-Δ strain. A darker exposure of pre-tRNAPro in the lhp1-Δ strain is shown in a separate panel (lane 16). WT, wild type.