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. 2002 Jul;22(14):5248–5256. doi: 10.1128/MCB.22.14.5248-5256.2002

TABLE 2.

Immunoprecipitation efficiency of Lhp1pa

RNA Value for strain (time in glucose):
GAL::lsm3 (0 h) Lhp1p- ProtA Lhp1p-ProtA/ GAL::lsm3 (0 h) Lhp1p-ProtA/ GAL::lsm3 (24 h)
scR1 0.2 44.2 30.3 0.4
Pre-RNase P RNA 0.2 15.6 6.4 0.35
RNase P RNA 0.2 1.4 0.85 0.6
U6 0.6 16.6 7.7 22.1
tRNATyr-PT 0.7 77.4 68.4 24.1
tRNA3Leu-PT 0.9 64.2 48.4 13.1
tRNA3Leu 0.1 0.4 0.3 0.1
Pre-5S 0.4 77.3 63 21
5S 0.5 3.9 2.4 1.1
a

Based on PhosphorImager quantification of Northern hybridization data from Fig. 4A to E. Numbers are expressed as percents, where supernatant plus pellet (P) equals 100%. The value for P was adjusted according to the amount of loaded RNA; see the legend to Fig. 4.