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. 2002 Jul;22(14):5141–5156. doi: 10.1128/MCB.22.14.5141-5156.2002

FIG. 3.

FIG. 3.

PRP4K is concentrated in SC35-containing splicing speckles. (A) Coimmunofluorescence with antibodies detecting PRP4K (H143) (FITC) and SC35 (Texas red) in human HT1080 cells alone, in the presence of actinomycin D (+Act D), or subjected to heat shock. PRP4K is nucleoplasmic but also concentrates in speckles that colocalize with SC35 in both transcriptionally active and transcriptionally inactive cells (i.e., heat shock and actinomycin D treated). (B) The RS-like domain of PRP4K is sufficient for localization to splicing speckles. HT1080 cells were transfected with either GFP-tagged murine PRP4K (GFP-PRP4K) or the RS domain of PRP4K fused to GFP (GFP-RS). Localization of GFP-tagged proteins was assessed by immunofluorescence in combination with the detection of endogenous SC35 (Texas red). GFP-tagged PRP4K forms distinct and large nuclear foci that colocalize with SC35. Scale bars, 5 μm.