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. 2002 Jul;22(14):5141–5156. doi: 10.1128/MCB.22.14.5141-5156.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 6. — In vitro kinase activity of PRP4K in immunoprecipitates. IPs with antibodies against PRP4K (MRC2 antibody [PRP4K-IP]), BRG1 (BRG1-IP), or PRP6 (PRP6-IP) were subjected to an in vitro kinase assay and resolved by SDS-PAGE, and phosphorylated proteins were visualized by autoradiography (Kinase Assay). Control reactions were carried out with either peptide-blocked MRC2 antibody (Pep. Block) or rabbit anti-sheep IgG (Mock). Western blot analysis of the PRP4K-IP complex was also carried out. Bands corresponding to known proteins are designated with arrows (arrow A, BRG1; arrow B, PRP4K; arrow C, PRP6). A contaminating band (arrow D) is present in the peptide-blocked kinase assay, and a strong band of ca. 54 kDa (arrow “?”) may correspond to phosphorylated IgG. In the PRP4K-IP, the strongly labeled band (arrow B) probably corresponds to autophosphorylated PRP4K.