FIG. 8.
Detection of the presence of PRP4K, BRG1, and PRP6 in the N-CoR-1 and -2 complexes. (A) Schematic representation of the purification of the N-CoR-1 and N-CoR-2 complexes (57). (B) Western blot analysis of the N-CoR-1 and N-CoR-2 complexes after elution from the N-CoR affinity column with antibodies against N-CoR, PRP4K, BRG1, and PRP6. The control lane is an aliquot from the mock affinity purification, and the input lane is the pooled S300 fractions containing N-CoR prior to immunoaffinity chromatography. Whereas BRG1 and PRP6 are components of both the N-CoR-1 and the N-CoR-2 complexes, PRP4K is primarily a component of the N-CoR-2 complex.