TABLE 1.
Replicative life spans of primary human cell lines engineered to evade p16INK4A-, p53-, and telomere length-dependent growth arrest mechanisms
| Cell line (cell type) | PD (change from control)a for cells transfected in the presence of:
|
|||||
|---|---|---|---|---|---|---|
| No vector | Empty vector | p53DD | cdk4R | p53DD and cdk4R | p53DD, cdk4R, and hTERT | |
| R2F (dermal fibroblast) | 80 | 74 (−6) | 123 (+43*) | 78 (−2) | 130 (+50*) | n.d. (imm. by hTERT alone) |
| HM-3 (peritoneal mesothelial cell) | 43 | 40 (−3) | 65 (+22*) | 40 (−3) | n.d. | n.d. (imm. by hTERT alone) |
| HuCL-22 (corneal keratinocyte) | 24 | 23 (−1) | 22 (−2) | 25 (+1) | 49 (+25*) | >86 (imm.) |
| Strain N (epidermal keratinocyte) | 55 | 51 (−1) | 64 (+9) | 63 (+8) | 98 (+43*) | >114 (imm.) |
| OKF4 (oral mucosal keratinocyte) | 37 | 32 (−5) | 32 (−5) | 42 (+5) | 61 (+24*) | >76 (imm.) |
| ConjEp-1 (conjunctival keratinocyte) | 18 | n.d. | 17 (−1) | n.d. | 33 (+15*) | >52 (imm.) |
| K107 (p16INK4A+/− epidermal keratinocyte) | 23 | 22 (−1) | 54 (+31*) (p16−/− variant) | 27 (+4) | 54 (+31*) | >67 (imm.) |
a
*, extended life span but not immortal; imm, immortalized; n.d., not determined.