Table 2.
The most abundant ORFs in the secretion stress related ESTs of T. reesei. 25 ORFs represented by the highest number of ESTs in the cDNA subtraction libraries or cDNA-AFLP fragments derived from the secretion stress conditions are listed. Gene names from T. reesei (small case letters) or S. cerevisiae (capital letters) are shown. Description and T. reesei protein model identifiers (ID) are from T. reesei genome version 1.2 website. The number of ESTs corresponding to the genes in the libraries are shown: "AFLP", fragments from cDNA-AFLP analysis induced in both tPA expression and DTT treatment; "DTThyb1", subtraction library from DTT treated culture after one subtraction round; "DTTHyb5", subtraction library from DTT treated culture after five subtraction rounds; "tPAhyb1", subtraction library from the tPA producing culture after one subtraction round; "tPAHyb5", subtraction library from tPA producing culture after five subtraction rounds.
| Gene | T. reesei protein Model ID | Description | AFLP | DTThyb1 | DTThyb5 | tPAhyb1 | tPAhyb5 | Total |
| TEF2 | tre41793 | translational elongation | 25 | 12 | 42 | 18 | 97 | |
| bip1 | tre42955 | HSP70 family ER chaperone | 1 | 55 | 1 | 1 | 58 | |
| tre42349 | posttranslational modification, protein turnover | 40 | 15 | 1 | 2 | 58 | ||
| UBI4 | tre45056 | posttranslational modification, protein turnover | 46 | 2 | 1 | 2 | 51 | |
| cbhII | tre44954 | cellobiohydrolase II | 40 | 10 | 50 | |||
| FRE4 | tre39576 | Ferric reductase-like transmembrane component | 26 | 7 | 33 | |||
| URA8 | tre13936 | phospholipid biosynthesis, pyrimidine base biosynthesis | 1 | 32 | 33 | |||
| cbhI | tre22421 | cellobiohydrolase I | 24 | 8 | 32 | |||
| cpc1 | tre37844 | basic-leucine zipper (bZIP) transcription factor | 21 | 10 | 31 | |||
| tre44646 | unknown | 13 | 12 | 1 | 1 | 27 | ||
| tre43768 | ankyrin | 13 | 11 | 2 | 26 | |||
| tre46310 | putative exoglucanase | 1 | 8 | 15 | 24 | |||
| ACT1 | tre41819 | actin | 8 | 2 | 8 | 5 | 23 | |
| ITR2 | tre43977 | intergal membrane myo-inositol transporter | 1 | 16 | 2 | 19 | ||
| tre45769 | aromatic compound metabolism | 19 | 19 | |||||
| tre43393 | ADP-ribosylglycohydrolase | 9 | 8 | 1 | 18 | |||
| prp2 | tre42899 | putative PDI-related protein | 2 | 3 | 13 | 18 | ||
| MBF1 | tre42770 | transcription factor MBF1 | 13 | 3 | 1 | 17 | ||
| HTA2 | tre35558 | histone H2A | 9 | 4 | 1 | 2 | 16 | |
| YLL023C | tre43299 | putative ER membrane protein | 10 | 5 | 15 | |||
| pdi1 | tre45146 | ER-resident foldase | 12 | 2 | 14 | |||
| cip1 | tre41957 | esterase homologue | 6 | 8 | 14 | |||
| tre45203 | Transcriptional coactivator p100 | 4 | 7 | 2 | 1 | 14 | ||
| hex1 | tre45186 | woronin body | 1 | 10 | 2 | 13 | ||
| VPS1 | tre44901 | vacuolar sorting protein | 12 | 1 | 13 | |||
| Amount of ESTs sequenced from the individual libraries | 94 | 961 | 240 | 510 | 242 | 2047 | ||