FIG. 2.

Dominant-negative BRG-1 can inhibit activation of MHC class II genes in HeLa cells. (A) HeLa cells were transfected with 20 ng of pCMV-CIITA and increasing amounts of pBJ5-BRG-1(K798R) (dominant-negative form of BRG-1) as indicated in the figure. The ratios of BRG-1 to CIITA are 1:1 (lane 3), 10:1 (lane 4), 20:1 (lane 5), 30:1 (lane 6), and 40:1 (lane 7). Total RNA was isolated, and Northern analysis was performed as described for Fig. 1A. (B) Band intensities of the blot shown in panel A were measured with QuantityOne software (Bio-Rad, Inc., Hercules, Calif.). The signal for HLA-DRA was normalized to the associated β-actin (β-act.) signal. The results obtained by transfection of pCMV-CIITA alone were set to 100.