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. 2002 Jul;22(14):5019–5026. doi: 10.1128/MCB.22.14.5019-5026.2002

FIG. 4.

FIG. 4.

BRG-1 and CIITA proteins coimmunoprecipitate. (A) COS-1 cells were transfected with pCMV-CIITA, pBJ5-BRG-1, or both plasmids. Anti-BRG-1 antiserum was used to immunoprecipitate (IP) BRG-1 and associated proteins from cellular lysates. Western blot (WB) analysis was performed with anti-HA antiserum, which recognizes the “flu tag” present on CIITA produced from pCMV-CIITA. The first lane contains cellular lysate from dually transfected cells. The band representing CIITA protein is indicated by an arrow. (B) Transfection was identical to that performed in panel A, but immunoprecipitation was performed with anti-HA antiserum and Western analysis was performed with anti-BRG-1 antiserum. The first lane contains cellular lysate from dually transfected cells. The band representing BRG-1 protein is shown. (C) The K798R mutation does not affect the interaction between BRG-1 and CIITA. The indicated expression plasmids were transfected into COS cells, and immunoprecipitation and Western blotting were performed with cellular lysates as in panel B.