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. 2002 Jul;22(14):5047–5053. doi: 10.1128/MCB.22.14.5047-5053.2002

FIG. 3.

FIG. 3.

Loss of YNG1 does not disrupt the NuA3 HAT complex. (A and B) Whole-cell extracts from the indicated strains were immunoprecipitated with αFLAG resin. The input whole-cell extracts and immunoprecipitates (IP) were Western blotted and probed for HA. (C) Whole-cell extracts from the indicated strains were resolved on a Superose 6 HR 10/30 column, and the fractions were assayed for the presence of Sas3HA3 by Western blotting. (D) Silver stained, SDS-PAGE (4% to 15% gradient) of NuA3 complexes purified from wild-type (YJW545) and yng1Δ (YJW546) strains. Bands corresponding to the verified subunits Sas3p and TAF30 are indicated. Contaminating bands are indicated by asterisks. IgG, immunoglobulin G.