FIG. 2.

Helix 1 of SF-1 contains an activation function AFH1 domain. (A) A schematic diagram of the major domains of the SF-1 protein is shown, with the amino acid sequence of the hinge-helix 1 region presented below. (B) Activities of constructs diagrammed in panel A are shown after cotransfection into NIH 3T3 cells with the luciferase reporter containing four tandem Gal4 binding sites, pGAL-RE-TK (54). Luciferase activity is expressed as fold activation, with the empty Gal4-DBD expression vector taken to be 1. To monitor for gross differences in expression levels of each Gal4 construct used, RT-PCR was performed using primers for both the Gal4 DBD present in all constructs and for actin; the results are shown in the lower panel for all corresponding constructs transfected (upper panel).