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. 2002 Oct;22(20):6946–6958. doi: 10.1128/MCB.22.20.6946-6958.2002

FIG. 2.

FIG. 2.

(A and B) An extra copy of RSP5 can partially suppress the growth defect caused by arp2 (A) and end3 (B). Temperature-sensitive arp2-1 and end3-1 strains were transformed with plasmids carrying RSP5 or rsp5-w genes or vector. (C) The arp2-1 strain was transformed with plasmid bearing UBI1 (RB2) or vector. Serial dilutions (1:10 steps) of transformants were spotted on YEPD and SC-Ura plates and allowed to grow at the indicated temperatures for 3 days. Growth was compared to that of the parental strain. (D) The end3-Δ strain was transformed with YCpHA-RSP5, YCpHA-rsp5-13, a plasmid bearing UBI1 (RB2), or vector. Transformants were replica plated on SC-Ura or YEPG and incubated at 30 or 34°C for 3 days.