FIG. 4.

RSP5 and rsp5-w2 suppress endocytosis defects of arp2-1 cells. The wild-type strain bearing vector (◊) or YCpHA-RSP5 (⧫) and the arp2-1 strain bearing vector (▪), YCpHA-RSP5 (▴), YCpHA-rsp5-w1 (*), YCpHA-rsp5-w2 (□), or YCpHA-rsp5-w3 (Δ) and plasmid p195gF were grown at 30°C with galactose as a carbon source and shifted to 34°C for 2 h. Cycloheximide (100 mg/ml) was then added to the media. Uracil uptake at the times indicated after addition of cycloheximide was measured. Representative results of three independent experiments are shown.