TABLE 1.
List of S. cerevisiae strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| T8-1D | MATα SUP11 ade2-1 mod5-1 ura3-1 lys2-1 leu2-3,112 his4-519 | 73 |
| TZ23 | MATα SUP11 rsp5-13 ade2-1 mod5-1 ura3-1 lys2-1 leu2-3,112 his4-519 | 72 |
| TZ81 | MATα SUP11 pan1-9 ade2-1 mod5-1 ura3-1 lys2-1 leu2-3,112 his4-519 | 73 |
| BG1 | MATα SUP11 rsp5-Δ ade2-1 mod5-1 ura3-1 lys2-1 leu2-3, 112 his4-519 p[HA-RSP5, CEN, LEU2] | 18 |
| BG2 | MATα SUP11 rsp5-Δ ade2-1 mod5-1 ura3-1 lys2-1 leu2-3, 112 his4-519 p[HA-rsp5-w1, CEN, LEU2] | 18 |
| BG3 | MATα SUP11 rsp5-Δ ade2-1 mod5-1 ura3-1 lys2-1 leu2-3, 112 his4-519 p[HA-rsp5-w2, CEN, LEU2] | 18 |
| BG4 | MATα SUP11 rsp5-Δ ade2-1 mod5-1 ura3-1 lys2-1 leu2-3, 112 his4-519 p[HA-rsp5-w3, CEN, LEU2] | 18 |
| T82-14C | MATa rsp5-13 SUP11 ade2-1 mod5-1 ura3-1 lys2-1 leu1 trp5 | This work |
| SS330 | MATa ade2-101 his3-200 tyr1 ura3-52 | 74 |
| ts817 | MATa ade2-101 his3-200 tyr1 ura3-52 sla2ts | 74 |
| RH144-3D | MATa his4 ura3 leu2 bar1-1 | 49 |
| RH266-1D | MATa his4 ura3 leu2 bar1-1 end3-1 | 49 |
| YPH500 | MATα ade2-101 his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 | 42 |
| YMW82 | MATα ade2-101 his3-Δ200, leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 arp2-1 | 42 |
| MK1 | MATα his3-Δ200 leu2-3, 112 ura3-52 lys2-801 trp1-1 RSP5::HA-RSP5 | M. Kwapisz, unpublished allele replacement |
| MK2 | MATα his3-Δ200 leu2-3, 112 ura3-52 lys2-801 trp1-1 rsp5::HA-rsp5-w1 | M. Kwapisz, unpublished allele replacement |
| MK4 | MATα his3-Δ200 leu2-3, 112 ura3-52 lys2-801 trp1-1 rsp5::HA-rsp5-w3 | M. Kwapisz, unpublished allele replacement |
| MK5 | MATα his3-Δ200 leu2-3, 112 ura3-5 lys2-801 trp1-1 rsp5::HA-rsp5-13 | M. Kwapisz, unpublished allele replacement |
| Y23725 | MAT a/α his3-Δ1/his3-Δ1 leu2-Δ0/leu2-Δ0 lys2-Δ0/LYS2 met15-Δ0/MET15 ura3-Δ0/ura3-Δ0 arp2-Δ(YDL029w::kan/MXY)/ARP2 | Euroscarf |
| Y129912 | MAT α his3-Δ1 leu2-Δ0 lys2-Δ0 ura3-Δ0 end3-Δ (YNL084c::kanMXY) | Euroscarf |