Skip to main content
. 2002 Oct;22(20):7024–7040. doi: 10.1128/MCB.22.20.7024-7040.2002

FIG. 2.

FIG. 2.

Ectopic expression of Ndt80 does not profoundly accelerate the induction of middle sporulation gene transcription. RNA samples (10 μg) obtained at the indicated time points from wild-type NDT80-expressing cells (A) or wild-type cells that also express NDT80 under the regulation of an IME2 promoter, IME2-NDT80 (B), were probed for the middle sporulation gene transcripts SPS1 and SPS2. RNA samples (10 μg) from a homozygous ime2 mutant expressing NDT80 from its endogenous promoter, NDT80 (C), an IME2 promoter, IME2-NDT80 (D), or a CUP1 promoter, CUP1-NDT80 (E), were probed for the middle sporulation gene transcripts SPS1 and SPS2. The ACT1 transcript served as a loading control.