FIG. 4.

Ime2 kinase accumulates early in meiosis and persists throughout MI and MII. (A) Ime2-Myc was immunoprecipitated at the indicated times from a culture of sporulating yeast cells, and the kinase activity was assayed by using histone H1 as a substrate. (B) Samples of the culture were stained with the DNA binding dye DAPI, and the number of cells that had completed MI was assayed by fluorescence microscopy. Two hundred cells were counted at each time point, and cells displaying more than one DAPI-staining body were counted as past MI.