FIG. 7.
Phosphorylation of Ndt80 by Ime2 is not required for Ndt80 to enter the nucleus. An NDT80-GFP fusion was expressed under the regulation of an IME2 promoter in an IME2/IME2 strain or an ime2/ime2 mutant strain or from a CUP1 promoter in an IME2/IME2 strain or an ime2/ime2 mutant strain. Samples of the cultures were taken at 0 and 4 h after induction of sporulation, and unfixed cells were examined by fluorescence microscopy to determine the localization of Ndt80-GFP fusions. Two hundred cells per sample were scored. CUP1-NDT80 was induced at time zero by addition of CuSO4 to a final concentration of 100 μM.