TABLE 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Relevant genotypea | Source |
|---|---|---|
| DSY1249 | a/α | This study |
| DSY1087 | a/α ime2::TRP1/me2::TRP1 | This study |
| DSY1312 | a/α URA3::IME2-NDT80HA/URA3::IME2-NDT80HA | This study |
| DSY1270 | a/α clb5::Kanr/clb5::Kanrclb6::TRP1/clb6::TRP1 URA3::IME2-NDT80HA/URA3::IME2-NDT80HA | This study |
| DSY1279 | a/α ime2::TRP1/ime2::TRP1 URA3::IME2-NDT80HA/URA3::IME2-NDT80HA | This study |
| DSY1289 | a/α URA3::CUP1-NDT80HA/URA3::CUP1-NDT80HA | This study |
| DSY1299 | a/α ime2::TRP1/ime2::TRP1 URA3::CUP1-NDT80HA/URA3::CUP1-NDT80HA | This study |
| DSY1303 | a/α ime2::TRP1/ime2::TRP1/ sic1::Kanr/sic1::KanrURA3::CUP1-NDT80HA/URA3::CUP1-NDT80HA | This study |
| DSY1257 | a/α ndt80::Kanr/ntd80::Kanr | This study |
| DSY1342 | a/α ndt80::Kanr/ndt80::Kanr | This study |
| DSY1344 | a/α ndt80::Kanr/ndt80::KanrURA3::IME2-NDT80HA/URA3::IME2-NDT80HA | This study |
| DSY1347 | a/α ime2::TRP1/ime2::TRP1 ndt80::Kanr/ndt80::KanrURA3::IME2-NDT80HA/URA3::IME-NDT80/IT"HA | This study |
| DSY1349 | a/α ndt80::Kanr/ndt80::KanrURA3::CUP1-NDT80HA/URA3::CUP1-NDT80HA | This study |
| DSY1352 | a/α ime2::TRP1/ime2::TRP1 ndt80::Kanr/ndt80::KanrURA3::CUP1-NDT80HA/URA3::CUP1-NDT80HA | This study |
| DSY1262 | a/α ime2::IME2-3×myc-LEU2/ime2::IME2-3×mye-LEU2 | This study |
| DSY1264 | a/α ime2::IME2Δ175-3×myc-LEU2/ime2::IME2Δ175-3×myc-LEU2 | This study |
| DSY1355 | a/α URA3::8×Lex°-LacZ/URA3::8×Lex°-LacZ | This study |
| DSY1357 | a/α ime2::TRP1/ime2::TRP1 URA3::8×Lex°-LacZ/URA3::8×Lex°-LacZ | This study |
| DSY1365 | a/α URA3::CUP1-NDT80-GFP/URA3::CUP1-NDT80GFP | This study |
| DSY1367 | a/α URA3::IME2-NDT80-GFP/URA3::IME2-NDT80-GFP | This study |
| DSY1369 | a/α ime2::TRP1/ime2::TRP1 URA3::CUP1-NDT80-GFP/URA3::CUP1-NDT80-GFP | This study |
| DSY1372 | a/α ime2::TRP1/ime2::TRP1 URA3::IME2-NDT80-GFP/URA3::IME2-NDT80-GFP | This study |
| PJ69-4A | atrp1 leu2 ura3 his3 gal4 gal80 LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL1-lacZ | P. James (21) |
a
All of the yeast strains listed, with the exception of PJ69-4A, are derived from the SK1 genetic background.