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. 2002 Oct;22(20):7242–7257. doi: 10.1128/MCB.22.20.7242-7257.2002

FIG. 8.

FIG. 8.

CRT inhibits translation of C/EBPα mRNA in cultured cells. (A) C/EBPα constructs used in these studies. Gal4-C/EBPα constructs lack the sequence that forms the 5′SL structure. N175 lacks both 5′SL and the C-terminal portion of C/EBPα containing region B. Gal4-C/EBPα-ΔB has an internal deletion and lacks the 5′SL and region B. DBD, coding sequence for the DNA binding domain. (B) HA-CRT inhibits translation of C/EBPα mRNAs containing the internal (SL int) structure. HA-CRT was cotransfected with C/EBPα constructs, and the proteins were isolated 18 h after translation and analyzed by Western blotting. Each filter was probed with Gal-4 or C/EBPα antibodies and then reprobed with antibodies to HA (top) and then with antibodies to β-actin to verify protein loading. The β-actin control is shown for the Gal4-C/EBPα membrane. A representative result of five independent experiments is shown. CRT represses translation of CMV-C/EBPα and Gal4-C/EBPα but does not inhibit translation of C/EBPα mRNAs lacking region B (Gal4-N175 and C/EBPα-ΔB).