Effect of atorvastatin on PAI-1, TSP-1, Ang-2, and VEGF-D in HUVECs. (A and B) Macroarray analysis demonstrated enhanced expression of Ang-2 and VEGF-D, and decreased PAI-1 and TSP-1, at 6 h after stimulation with atorvastatin. (results of two independent hybridizations are shown). (C) RTPCR confirmed the induction of VEGF-D; however, this effect was visible only after 38 cycles of amplification. Ang-2 expression was detected after 30 cycles.