FIG. 3.

EMSA using recombinant DNA-binding domains or proteins. Analysis of the involvement of Xyr1 (A, B), Ace2 (C), Ace1 (D), and Hap2/3/5 (E) in binding to the xyn1 5′ noncoding region. The respectively labeled oligonucleotides used are described in detail in Table 1. Thrombin-cleaved fusion protein (0.2 μg, corresponding molar ratio is approximately 1:20 between DNA and recombinant protein) was used, if not indicated differently: panel A, lanes 2, 3, and 4, 0.05, 0.1, and 0.2 μg recombinant protein; lane 5, 0.2 μg uncleaved Xyr1::Gst fusion protein. −, free probe; +, addition of the respective thrombin-cleaved fusion proteins to the binding reaction.