TABLE 1.
Name | Sequence (5′-3′)a | Position (promoter or gene)b |
---|---|---|
Xyn1F-vivo1 | CTCAAGCAACTACGTAAAACTCCATG | −524 to −499 (xyn1 promoter) |
Xyn1F-vivo2 | CACTGGAATACAACATCCTCCGCAAG | −481 to −456 (xyn1 promoter) |
Xyn1F-vivo3 | GGAATACAACATCCTCCGCAAGTCCGAC | −477 to −450 (xyn1 promoter) |
Xyn1R-vivo1 | ATAGATTGAACGCCACCGCAATATC | −276 to −300 (xyn1 promoter) |
Xyr1R-vivo2 | TATGGCAGTAATGCATCGAACCCGG | −332 to −356 (xyn1 promoter) |
Xyr1R-vivo3 | GCAGTAATGCAATCGAACCCGGCGCTT | −336 to −361 (xyn1 promoter) |
Xyr1-4f | ACIGAC/TGAC/TGTIGTIACITAC/TATA/C/TCA | (xyr1 gene) |
Xyr1-7r | TAA/G/TATICCA/GAAA/GAAA/GAAIGGCATA/GAA | (xyr1 gene) |
xyn1outf | GTCGAGGTCGACGCAAATGG | −538 to −526 (xyn1 promoter) |
xyn1outr | GTGAGTTCAGGCTTTTTCATCTAGAGATG | 20 to 1 (hph gene) |
xyr1M1f | GATTGGCAGTCTAAATGCGACATCTT | −445 to −420 (xyn1 promoter) |
xyr1M1r | AAGATGTCGCATTTAGACTGCCAATC | −420 to −445 (xyn1 promoter) |
xyr1M2f | GCGACATCTTAGACGGATGCAC | −429 to −408 (xyn1 promoter) |
xyr1M2r | GTGCATCCGTCTAAGATGTCGC | −408 to −429 (xyn1 promoter) |
xyr1M3f | GCCCCTTGACTTGAAAGGCAGGCT | −457 to −434 (xyn1 promoter) |
xyr1M3r | AGCCTGCCTTTCAAGTCAAGGGGC | −434 to −457 (xyn1 promoter) |
xyr1Exf | CGAGGATCCGAGCTTTCGAGTTCACGCATG | 163 to 183 (xyr1 gene) |
xyr1Exr | TTTCTCGAGCTCAATGTGGCCATGAG | 651 to 635 (xyr1 gene) |
Prxyn1.1f | TTGGCAGGCTAAATGCGACATCTTAGCCGGA | −430 to −400 (xyn1 promoter) |
Prxyn1.1r | TGCATCCGGCTAAGATGTCGCATTTAGCCTG | −396 to −426 (xyn1 promoter) |
Prxyn1.1M1f | TTGGCAGTCTAAATGCGACATCTTAGCCGGA | −430 to −400 (xyn1 promoter) |
Prxyn1.1M1r | TGCATCCGGCTAAGATGTCGCATTTAGACTG | −396 to −426 (xyn1 promoter) |
Prxyn1.1M2f | TTGGCAGGCTAAATGCGACATCTTAGACGGA | −430 to −400 (xyn1 promoter) |
Prxyn1.1M2r | TGCATCCGTCTAAGATGTCGCATTTAGCCTG | −396 to −426 (xyn1 promoter) |
Prxyn1.2f | CCCCTTGACTTGATTGGCAGGC | −443 to −422 (xyn1 promoter) |
Prxyn1.2r | GGGAGCCTGCCAATCAAGTCAA | −418 to −439 (xyn1 promoter) |
Prxyn1.2M3f | CCCCTTGACTTGAAAGGCAGGC | −443 to −422 (xyn1 promoter) |
Prxyn1.2M3r | GGGAGCCTGCCTTTCAAGTCAA | −418 to −439 (xyn1 promoter) |
Prcbh1f | ATATACCAGCGGCTAATAATTG | −789 to −768 (cbh1 promoter) |
Prcbh1r | TGTACAATTATTAGCCGCTGGT | −764 to −785 (cbh1 promoter) |
Prcbh1mut2D2f | ATATACCAGCTTATAATAATTG | −789 to −768 (cbh1 promoter) |
Prcbh1mut2D2r | TGTACAATTATTATAAGCTGGT | −764 to −785 (cbh1 promoter) |
Letters underlined by single lines indicate identified regulatory elements, double lines mark introduced restriction sites. Bold characters indicate mutations within identified motifs.
Positions of the oligonucleotides in the respective promoters or genes are given.