TABLE 1.
Primers used in this study
| Name | Sequence (5′-3′)a | Position (promoter or gene)b |
|---|---|---|
| Xyn1F-vivo1 | CTCAAGCAACTACGTAAAACTCCATG | −524 to −499 (xyn1 promoter) |
| Xyn1F-vivo2 | CACTGGAATACAACATCCTCCGCAAG | −481 to −456 (xyn1 promoter) |
| Xyn1F-vivo3 | GGAATACAACATCCTCCGCAAGTCCGAC | −477 to −450 (xyn1 promoter) |
| Xyn1R-vivo1 | ATAGATTGAACGCCACCGCAATATC | −276 to −300 (xyn1 promoter) |
| Xyr1R-vivo2 | TATGGCAGTAATGCATCGAACCCGG | −332 to −356 (xyn1 promoter) |
| Xyr1R-vivo3 | GCAGTAATGCAATCGAACCCGGCGCTT | −336 to −361 (xyn1 promoter) |
| Xyr1-4f | ACIGAC/TGAC/TGTIGTIACITAC/TATA/C/TCA | (xyr1 gene) |
| Xyr1-7r | TAA/G/TATICCA/GAAA/GAAA/GAAIGGCATA/GAA | (xyr1 gene) |
| xyn1outf | GTCGAGGTCGACGCAAATGG | −538 to −526 (xyn1 promoter) |
| xyn1outr | GTGAGTTCAGGCTTTTTCATCTAGAGATG | 20 to 1 (hph gene) |
| xyr1M1f | GATTGGCAGTCTAAATGCGACATCTT | −445 to −420 (xyn1 promoter) |
| xyr1M1r | AAGATGTCGCATTTAGACTGCCAATC | −420 to −445 (xyn1 promoter) |
| xyr1M2f | GCGACATCTTAGACGGATGCAC | −429 to −408 (xyn1 promoter) |
| xyr1M2r | GTGCATCCGTCTAAGATGTCGC | −408 to −429 (xyn1 promoter) |
| xyr1M3f | GCCCCTTGACTTGAAAGGCAGGCT | −457 to −434 (xyn1 promoter) |
| xyr1M3r | AGCCTGCCTTTCAAGTCAAGGGGC | −434 to −457 (xyn1 promoter) |
| xyr1Exf | CGAGGATCCGAGCTTTCGAGTTCACGCATG | 163 to 183 (xyr1 gene) |
| xyr1Exr | TTTCTCGAGCTCAATGTGGCCATGAG | 651 to 635 (xyr1 gene) |
| Prxyn1.1f | TTGGCAGGCTAAATGCGACATCTTAGCCGGA | −430 to −400 (xyn1 promoter) |
| Prxyn1.1r | TGCATCCGGCTAAGATGTCGCATTTAGCCTG | −396 to −426 (xyn1 promoter) |
| Prxyn1.1M1f | TTGGCAGTCTAAATGCGACATCTTAGCCGGA | −430 to −400 (xyn1 promoter) |
| Prxyn1.1M1r | TGCATCCGGCTAAGATGTCGCATTTAGACTG | −396 to −426 (xyn1 promoter) |
| Prxyn1.1M2f | TTGGCAGGCTAAATGCGACATCTTAGACGGA | −430 to −400 (xyn1 promoter) |
| Prxyn1.1M2r | TGCATCCGTCTAAGATGTCGCATTTAGCCTG | −396 to −426 (xyn1 promoter) |
| Prxyn1.2f | CCCCTTGACTTGATTGGCAGGC | −443 to −422 (xyn1 promoter) |
| Prxyn1.2r | GGGAGCCTGCCAATCAAGTCAA | −418 to −439 (xyn1 promoter) |
| Prxyn1.2M3f | CCCCTTGACTTGAAAGGCAGGC | −443 to −422 (xyn1 promoter) |
| Prxyn1.2M3r | GGGAGCCTGCCTTTCAAGTCAA | −418 to −439 (xyn1 promoter) |
| Prcbh1f | ATATACCAGCGGCTAATAATTG | −789 to −768 (cbh1 promoter) |
| Prcbh1r | TGTACAATTATTAGCCGCTGGT | −764 to −785 (cbh1 promoter) |
| Prcbh1mut2D2f | ATATACCAGCTTATAATAATTG | −789 to −768 (cbh1 promoter) |
| Prcbh1mut2D2r | TGTACAATTATTATAAGCTGGT | −764 to −785 (cbh1 promoter) |
a
Letters underlined by single lines indicate identified regulatory elements, double lines mark introduced restriction sites. Bold characters indicate mutations within identified motifs.
b
Positions of the oligonucleotides in the respective promoters or genes are given.